Journal article
Variation in the Toll-like Receptor 4 (TLR4) gene affects milk traits in dairy cows
H Zhou, L Cheng, H Gong, SO Byun, GR Edwards, JGH Hickford
Journal of Dairy Research | CAMBRIDGE UNIV PRESS | Published : 2017
Abstract
The objective of this Research Communication was to use polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) analysis to investigate a region of the bovine TLR4 gene (TLR4) in pasture-fed New Zealand (NZ) Holstein-Friesian × Jersey (HF × J) cross dairy cows and to determine whether gene variation was associated with milk production traits. Genetic variation was observed, with two variants (A and B) containing a single nucleotide polymorphism (SNP) (c.2021C/T) that was non-synonymous and putatively results in a p.Thr674Ile substitution in the transmembrane/cytoplasmic domain of TLR4. Variant A was associated with higher milk yields, but lower milk fat percentages, ..
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Awarded by Ministry of Research, Innovation and Science
Funding Acknowledgements
Financial support from the NZ Ministry of Science and Innovation (DRCX 0802) and Lincoln University Gene-Marker Laboratory are acknowledged. We acknowledge the support of AGMARDT for Long Cheng, and thank Innocent Rugoho, Helen Hague, Thomas Vazhathara and Freeman Fang for technical support. We thank Peter Hancox from the Lincoln University Dairy Farm for assistance with sampling.