Journal article

Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in frozen human plasma

JA Bowden, A Heckert, CZ Ulmer, CM Jones, JP Koelmel, L Abdullah, L Ahonen, Y Alnouti, AM Armando, JM Asara, T Bamba, JR Barr, J Bergquist, CH Borchers, J Brandsma, SB Breitkopf, T Cajka, A Cazenave-Gassiot, A Checa, MA Cinel Show all

Journal of Lipid Research | Published : 2017

Open access

Abstract

As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950- Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each laboratory using a different lipidomics workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detecte..

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University of Melbourne Researchers

Grants

Awarded by National Science Foundation


Funding Acknowledgements

The work performed in this study would not be possible without the funding support (either partial or full) for each contributing laboratory. At the request of these funded laboratories, we would like to acknowledge the following grant support statements: grants from the National Institutes of Health [including Lipid Metabolites and Pathways Strategy (LIPID MAPS)] U54 GM069338, R01 GM20501-41, and P30 DK064391 (E.A.D., O.Q.); the Research Center for Transomics Medicine, Kyushu University, supported by grants from ALCA, AMED-CREST, and Japan Science and Technology Agency (JPMJCR1395) (T.B., Y.I., H.T.); Natural Sciences and Engineering Research Council of Canada (C.M., P.R., D.V.); Swedish Research Council Grant 2015-4870 (J.Be.); National Center for Advancing Translational Sciences Grant UL1 TR000040 (Hon. J.,R.N., S.C.); Foundation for the National Institutes of Health Grants 5P01CA120964 and 5P30CA006516 (J.M.A., S.B. B.,M. Y.); National Institute of General Medical Sciences Grant PO1 GM095467 (R.A.C., C.N.S.); National Center for Research Resources Grant S10RR027926 (K.R.M., S.Z.); National Institutes of Health Grants P20 HL113452 and U24 DK097154 (T.C., O.F.); Southeast Center for Integrated Metabolomics (SECIM) via National Institutes of Health Grant U24 DK097209 (R.Pa., D.M., T.J.G., J.P.K.); Austrian Science Fund (FWF) Grant P26148-N19 (H.C.K., M.T.,A. T.); the Southampton Centre for Biomedical Research (SCBR)/NIHR Southampton Respiratory Biomedical Research Unit (G.K., A.D.P., J.Br.); the Metabolomics Innovation Centre (TMIC) through the Genome Innovations Network (GIN) funding from Genome Canada, Genome Alberta, and Genome British Columbia for operations (205MET and 7203) and technology development (215MET and MC3T) (J.H., K.L., C.H.B.); Foundation for the National Institutes of Health Grant P01 HL034300 (Mass Spectrometry Core) (M.L.S., K.H.G., Hou. J.); Canadian Institutes of Health Research Grant FDN143309 and Canadian Foundation for Innovation Grant CFI 12156 (M.P., M.L., D.R.); the Kansas Lipidomics Research Center (KLRC), supported by National Science Foundation Grants (MCB 1413036, MCB 0920663, DBI 0521587, and DBI 1228622), Kansas INBRE (Foundation for the National Institutes of Health Grant P20 RR16475 from the INBRE Program of the National Center for Research Resources), NSF EPSCoR Grant EPS-0236913, Kansas Technology Enterprise Corporation, and Kansas State University (R.W., M.R.R., L.Y.); Swedish Heart-Lung Foundation Grants HLF 20140469 and HLF 20150640 (C.E.W., J.K., A.F., A.C.); the Stable Isotope and Metabolomics Core Facility of the Diabetes Research and Training Center (DRTC) of the Albert Einstein College of Medicine, supported by National Institutes of Health/NCI Grant P60DK020541 (X.H.Z., Y.Q., and I. J.K.); Biomarkers Core Laboratory, Irving Institute for Clinical and Translational Research, and Columbia University Medical Center acknowledge support from the National Center for Advancing Translational Sciences, National Institutes of Health Grant UL1 TL001873); the National University of Singapore through Life Sciences Institute and the Yong Loo Lin School of Medicine Department of Biochemistry (A.C-G., F.T., M.R.W.) and the Department of Biological Sciences (A.H.N.); and Singapore National Research Foundation Grant NRFI2015-05 (M.R.W.). Additional support was provided by the Leading Edge Endowment Fund (University of Victoria), the Segal McGill Chair in Molecular Oncology at McGill University (Montreal, Quebec, Canada), the Warren Y. Soper Charitable Trust, and the Alvin Segal Family Foundation to the Jewish General Hospital (Montreal, Quebec, Canada) (C.H.B). Certain commercial equipment, instruments, or materials are identified in this paper to adequately specify the experimental procedures. Such identification does not imply recommendation or endorsement by the National Institute of Standards and Technology; nor does it imply that the materials or equipment identified are necessarily the best for the purpose. Furthermore, the content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Standards and Technology, the National Institutes of Health, or any of the participating organizations.