Journal article
Identification of novel human NK cell progenitor subsets
P Sathe, SHM Pang, R Delconte, N Elwood, ND Huntington
International Journal of Molecular Sciences | MDPI | Published : 2017
DOI: 10.3390/ijms18122716
Open access
Abstract
Understanding the pathways and regulation of human haematopoiesis, in particular, lymphopoiesis, is vital to manipulation of these processes for therapeutic purposes. However, although haematopoiesis has been extensively characterised in mice, translation of these findings to human biology remains rudimentary. Here, we describe the isolation of three progenitor subsets from human foetal bone marrow that represent differential stages of commitment to the natural killer (NK) cell lineage based on IL-15 responsiveness. We identify CD7 as a marker of IL-15 responsive progenitors in human bone marrow and find that this expression is maintained throughout commitment and maturation. Within the CD7+..
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Awarded by Cancer Research Institute
Funding Acknowledgements
We acknowledge the Bloemenhove Clinic (Heemstede, The Netherlands) for providing foetal tissues and Hergen Spits (AMC, Amsterdam-Zuidoost, The Netherlands) for the kind gift of foetal bone marrow and reagents. This work is supported project grants from the National Health and Medical Research Council (NHMRC) of Australia (1049407, 1066770, 1057852, 1027472 to N.D.H), an NHMRC CDF2 fellowship (NDH) as well as an NHMRC Independent Research Institute Infrastructure Support scheme grant and a Victorian State Government Operational Infrastructure Scheme grant. NDH is a recipient of research grants from the following organisations: Ian Potter Foundation (Australia), Harry J Lloyd Charitable Trust (USA), Melanoma Research Alliance (USA) and Cancer Research Institute (USA).