Comparative study of three methods for detecting avian leukosis viruses.
EJ Smith, LB Crittenden, J Ignjatovic
Infection and Immunity | Published : 1977
This investigation was designed to compare detection limits for avian leukosis viruses after infection of chicken fibroblasts with decimal dilutions of Rous-associated virus type 1 (RAV-1). At 5, 9, 14, and 19 days postinfection, cells were examined for group-specific (gs) antigens by microtiter complement-fixation (CF) tests for avian leukosis viruses and by radioimmunoassays (RIA) for the major gs antigen having a molecular weight of 27,000 (p27). Culture fluids, collected at the same time periods, were also assayed for reverse transcriptase activities. We found that minimally infected cultures expressed virus proteins within 9 days postinfection regardless of method used. Although p27 RIA..View full abstract