Characterizing glycoproteins by mass spectrometry in Campylobacter jejuni

Abstract

The glycosylation systems of Campylobacter jejuni (C. jejuni) are considered archetypal examples of both N-and O-linked glycosylations in the field of bacterial glycosylation. The discovery and characterization of these systems both have revealed important biological insight into C. jejuni and have led to the refinement and enhancement of methodologies to characterize bacterial glycosylation. In general, mass spectrometrybased characterization has become the preferred methodology for the study of C. jejuni glycosylation because of its speed, sensitivity, and ability to enable both qualitative and quantitative assessments of glycosylation events. In these experiments the generation of insight..