Immunoprecipitation of Specific Polysomes Using Staphylococcus aureus: Purification of the Immunoglobulin k Chain Messenger RNA from the Mouse Myeloma MPC11

Abstract

A modified procedure for immunoprecipitation of specific polysomes (Mueller-Lantzsch, N., & Fan, H. (1976) Cell 9, 579) has been examined as a method for purification of specific mRNAs. Antibody-polysome complexes were recovered by adsorption to inactivated Staphylococcus aureus which has a cell wall component (protein A) with a high affinity for immunoglobulins. The technique was evaluated by precipitation of polysomes synthesizing immunoglobulin k chains from mouse myelomas. Under the immunoprecipitation conditions used neither affinity purified antibody nor formaldehyde-fixed staphylococci degraded polysomes. Using purified sheep anti-mouse immunoglobulin, 10-15% of the polysomes from the..