Journal article
Metabolomic profiles of a midge (Procladius villosimanus, kieffer) are associated with sediment contamination in urban wetlands
KJ Jeppe, KA Kouremenos, KR Townsend, DF Macmahon, D Sharley, DL Tull, AA Hoffmann, V Pettigrove, SM Long
Metabolites | MDPI | Published : 2017
Abstract
Metabolomic techniques are powerful tools for investigating organism-environment interactions. Metabolite profiles have the potential to identify exposure or toxicity before populations are disrupted and can provide useful information for environmental assessment. However, under complex environmental scenarios, metabolomic responses to exposure can be distorted by background and/or organismal variation. In the current study, we use LC-MS (liquid chromatography-mass spectrometry) and GC-MS (gas chromatography-mass spectrometry) to measure metabolites of the midge Procladius villosimanus inhabiting 21 urban wetlands. These metabolites were tested against common sediment contaminants using rand..
View full abstractGrants
Funding Acknowledgements
The authors thank Melbourne Water for funding this research and the researchers at CAPIM for their help collecting samples, running bioassays and commenting on the manuscript. We are also grateful to the Chemistry Department at The University of Melbourne for performing the GC-MS analysis of chemicals in the sediment samples and the staff at Metabolomics Australia for performing the GC-MS and LC-MS metabolomic analysis.