Journal article
Lipid droplet remodelling and reduced muscle ceramides following sprint interval and moderate-intensity continuous exercise training in obese males
SO Shepherd, M Cocks, PJ Meikle, NA Mellett, AM Ranasinghe, TA Barker, AJM Wagenmakers, CS Shaw
International Journal of Obesity | NATURE PUBLISHING GROUP | Published : 2017
DOI: 10.1038/ijo.2017.170
Abstract
Background/Objectives:In obesity, improved muscle insulin sensitivity following exercise training has been linked to the lowering of diacylglycerol (DAG) and ceramide concentrations. Little is known, however, about how improved insulin action with exercise training in obese individuals relates to lipid droplet (LD) adaptations in skeletal muscle. In this study we investigated the hypothesis that short-term sprint interval training (SIT) and moderate-intensity continuous training (MICT) in obese individuals would increase perilipin (PLIN) expression, increase the proportion of LDs in contact with mitochondria and reduce muscle concentrations of DAGs and ceramides.Methods:Sixteen sedentary obe..
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Funding Acknowledgements
The antibody against myosin (human slow twitch fibres, A4.840) used in the study was developed by Dr Blau and obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA 52242. This work was supported by a grant from the Insulin Dependent Diabetes Trust.