Journal article
Immunoglobulin G1 allotype influences antibody subclass distribution in response to HIV gp140 vaccination
S Kratochvil, PF McKay, AW Chung, SJ Kent, J Gilmore, RJ Shattock
Frontiers in Immunology | FRONTIERS MEDIA SA | Published : 2017
Abstract
Antibody subclasses exhibit extensive polymorphisms (allotypes) that could potentially impact the quality of HIV-vaccine induced B cell responses. Allotypes of immunoglobulin (Ig) G1, the most abundant serum antibody, have been shown to display altered functional properties in regard to serum half-life, Fc-receptor binding and FcRn-mediated mucosal transcytosis. To investigate the potential link between allotypic IgG1-variants and vaccine-generated humoral responses in a cohort of 14 HIV vaccine recipients, we developed a novel protocol for rapid IgG1-allotyping. We combined PCR and ELISA assays in a dual approach to determine the IgG1 allotype identity (G1m3 and/or G1m1) of trial participan..
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Grants
Awarded by Horizon 2020 Framework Programme
Funding Acknowledgements
This study was funded by the International AIDS Vaccine Initiative (IAVI), and conduct of the clinical study was supported by the NIHR at Imperial College Healthcare NHS Trust. Provision of CN54gp140 and GLA-AF was supported through core funding from the Wellcome Trust via UKHVC (083844/Z/07/Z). We gratefully acknowledge Dormeur Investment Service Ltd. for providing funds to purchase equipment used in these studies. The Fc-functional antibody work was funded by the European Union's Horizon 2020 research and innovation program under grant agreement no. 681032 and an Australian NHMRC-EU collaborative grant #1115828.