Biocompatibility and Osteogenic/Calcification Potential of Casein Phosphopeptide-amorphous Calcium Phosphate Fluoride

Abstract

Introduction: Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and CPP-ACP with fluoride (CPP-ACFP) have been shown to provide bioavailable ions to promote mineralization. Hence, the aim of this study was to evaluate the materials’ biocompatibility and osteogenic/calcification potential for endodontic applications. Methods: Human and mouse osteoblast-like and fibroblast-like cell lines were incubated with 0.05%–3.0% w/v CPP-ACP and CPP-ACFP, and toxicity, proliferation, alkaline phosphatase, interleukin (IL)-1α and IL-6 production, collagen type I, osteocalcin, and osteopontin production, and mineralization/calcification were determined. Results: CPP-ACP and CPP-ACFP were non-toxi..