Journal article

Three UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis

Xianhai Zhao, Nian Liu, Na Shang, Wei Zeng, Berit Ebert, Carsten Rautengarten, Qing-Yin Zeng, Huiling Li, Xiaoyang Chen, Cherie Beahan, Antony Bacic, Joshua L Heazlewood, Ai-Min Wu

JOURNAL OF EXPERIMENTAL BOTANY | OXFORD UNIV PRESS | Published : 2018

Abstract

UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs). The Arabidopsis genome encodes six UXSs, which fall into two groups based upon their subcellular location: the Golgi lumen and the cytosol. The latter group appears to play an important role in xylan biosynthesis. Cytosolic UDP-Xyl is transported into the Golgi lumen by three UDP-Xyl transporters (UXT1, 2, and 3). However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition. This prompted us to further investigate ..

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Grants

Awarded by National Key Research and Development Program of China


Awarded by National Natural Science Foundation of China


Awarded by Guangdong Province Science and Technology Project


Awarded by Australian Research Council Future Fellowship


Awarded by Australia Research Council


Funding Acknowledgements

Arabidopsis mutants were obtained from the ABRC at Ohio State University (www. arabidopsis. org). This work was supported by the National Key Research and Development Program of China (grant no. 2016YFD0600105), the National Natural Science Foundation of China (grant no. 31670670 and 31170165), the Guangdong Province Science and Technology Projects (grant no. 2015A050502045 and 2016A010104012) and Open Fund of State Key Laboratory of Tree Genetics and Breeding (Beijing). JLH and BE are supported by Australian Research Council Future Fellowships (grant no. FT130101165 and FT160100276). AB acknowledges support from the Australia Research Council to the ARC Centre of Excellence in Plant Cell Walls (grant no. CE1101007).