Journal article

The thermodynamics of Pr55(Gag)-RNA interaction regulate the assembly of HIV

Hanumant S Tanwar, Keith K Khoo, Megan Garvey, Lynne Waddington, Andrew Leis, Marcel Hijnen, Tony Velkov, Geoff J Dumsday, William J McKinstry, Johnson Mak

PLOS PATHOGENS | PUBLIC LIBRARY SCIENCE | Published : 2017

Abstract

The interactions that occur during HIV Pr55Gag oligomerization and genomic RNA packaging are essential elements that facilitate HIV assembly. However, mechanistic details of these interactions are not clearly defined. Here, we overcome previous limitations in producing large quantities of full-length recombinant Pr55Gag that is required for isothermal titration calorimetry (ITC) studies, and we have revealed the thermodynamic properties of HIV assembly for the first time. Thermodynamic analysis showed that the binding between RNA and HIV Pr55Gag is an energetically favourable reaction (ΔG<0) that is further enhanced by the oligomerization of Pr55Gag. The change in enthalpy (ΔH) widens sequen..

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Grants

Awarded by Australian National Health and Medical Research Council


Awarded by National Institute of Health NIH


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Funding Acknowledgements

JM is recipient of funding from Australian National Health and Medical Research Council project grants App543107 and App1121697, and is a sub-awardee of National Institute of Health NIH RO1 GM064347. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.