Journal article
Probes to monitor activity of the paracaspase MALT1
J Hachmann, LE Edgington-Mitchell, M Poreba, LE Sanman, M Drag, M Bogyo, GS Salvesen
Chemistry and Biology | CELL PRESS | Published : 2015
Abstract
The human paracaspase mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) plays a central role in nuclear factor-κB (NF-κB) signaling as both a protease and scaffolding protein. Knocking out MALT1 leads to impaired NF-κB signaling and failure to mount an effective immune response. However, it is unclear to which degree it is the scaffolding function versus the proteolytic activity of MALT1 that is essential. Previous work involving a MALT1 inhibitor with low selectivity suggests that the enzymatic function plays an important role in different cell lines. To help elucidate this proteolytic role of MALT1, we have designed activity-based probes that inhibit its proteolyti..
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Awarded by National Institutes of Health
Funding Acknowledgements
We thank Daniel Krappmann and John C. Reed for supplying reagents. We also thank Khatereh Motamedchaboki (Sanford-Burnham Medical Research Institute, Proteomics Facility), supported by NIH grant P30 CA030199, for help with mass spectrometry. This research was supported by NIH grants R01-GM09040 and R01 CA163743 to G.S.S. and R01 EB005011 and R01 HL11630703 to M.B. M.D. received support from the Foundation for Polish Science and a statutory activity subsidy from the Polish Ministry of Science and Higher Education for the Faculty of Chemistry at Wroclaw University of Technology.