A new panel of epitope mapped monoclonal antibodies recognising the prototypical tetraspanin CD81.
Joe Grove, Ke Hu, Michelle J Farquhar, Margaret Goodall, Lucas Walker, Mohammed Jamshad, Heidi E Drummer, Roslyn M Bill, Peter Balfe, Jane A McKeating
Wellcome Open Research | Published : 2017
Background: Tetraspanins are small transmembrane proteins, found in all higher eukaryotes, that compartmentalize cellular membranes through interactions with partner proteins. CD81 is a prototypical tetraspanin and contributes to numerous physiological and pathological processes, including acting as a critical entry receptor for hepatitis C virus (HCV). Antibody engagement of tetraspanins can induce a variety of effects, including actin cytoskeletal rearrangements, activation of MAPK-ERK signaling and cell migration. However, the epitope specificity of most anti-tetraspanin antibodies is not known, limiting mechanistic interpretation of these studies. Methods: We generated a panel of monoclo..View full abstract