Conference Proceedings
Population Dynamics of Ammonia Oxidizing Bacteria and Archaea and Relationships with Nitrification Rate in New Zealand Grazed Grassland Soils
Hong Jie Di, Keith C Cameron, Jupei Shen, Jizheng He, Chris S Winfield, Maureen O'Callaghan, Saman Bowatte, JM Xu (ed.), PM Huang (ed.)
MOLECULAR ENVIRONMENTAL SOIL SCIENCE AT THE INTERFACES IN THE EARTH'S CRITICAL ZONE | SPRINGER-VERLAG BERLIN | Published : 2010
Abstract
The oxidation of ammonia (NH3) to nitrate (NO3−) in different ecosystems is a key process in the global nitrogen (N) cycle which has major ecological and environmental implications both in influencing nitrous oxide (N2O) emissions and nitrate leaching. We investigated the population dynamics of ammonia oxidising bacteria (AOB) and ammonia oxidising archaea (AOA) under controlled laboratory conditions using quantitative polymerase chain reaction (qPCR or real-time PCR) in six different intensively managed dairy grassland soils sampled from across New Zealand. The AOA amoA gene copy numbers varied from 1.27×107 to 3.85×106. The AOA to AOB ratio varied from 10.7 to 0.2. While the AOB population..
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Funding Acknowledgements
We would like to thank the New Zealand Foundation for Research, Science and Technology (FRST)for funding; Drs Ross Monaghan, Stewart Ledgard and Mark Sheppard of AgResearch and Dr Bruce Thorrold and Deanne Waugh of Dairy NZ for assistance with soil sampling, Emily Gerard and Shona Brock of AgResearch and Jie Lei, Steve Moore, Carole Barlow, and Trevor Hendry of Lincoln University, for technical support.