Loss-of-Function Mutation of REDUCED WALL ACETYLATION2 in Arabidopsis Leads to Reduced Cell Wall Acetylation and Increased Resistance to Botrytis cinerea
Yuzuki Manabe, Majse Nafisi, Yves Verhertbruggen, Caroline Orfila, Sascha Gille, Carsten Rautengarten, Candice Cherk, Susan E Marcus, Shauna Somerville, Markus Pauly, J Paul Knox, Yumiko Sakuragi, Henrik Vibe Scheller
Plant Physiology | AMER SOC PLANT BIOLOGISTS | Published : 2011
Nearly all polysaccharides in plant cell walls are O-acetylated, including the various pectic polysaccharides and the hemicelluloses xylan, mannan, and xyloglucan. However, the enzymes involved in the polysaccharide acetylation have not been identified. While the role of polysaccharide acetylation in vivo is unclear, it is known to reduce biofuel yield from lignocellulosic biomass by the inhibition of microorganisms used for fermentation. We have analyzed four Arabidopsis (Arabidopsis thaliana) homologs of the protein Cas1p known to be involved in polysaccharide O-acetylation in Cryptococcus neoformans. Loss-of-function mutants in one of the genes, designated REDUCED WALL ACETYLATION2 (RWA2)..View full abstract
Awarded by U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research
This work was supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research (contract no. DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U. S. Department of Energy) and by the Danish Natural Science Research Council. C. C. was supported in part by a Department of Energy grant to S. S.