Journal article

Role of mitochondrial inner membrane organizing system in protein biogenesis of the mitochondrial outer membrane

Maria Bohnert, Lena-Sophie Wenz, Ralf M Zerbes, Susanne E Horvath, David A Stroud, Karina von der Malsburg, Judith M Mueller, Silke Oeljeklaus, Inge Perschil, Bettina Warscheid, Agnieszka Chacinska, Marten Veenhuis, Ida J van der Klei, Guenther Daum, Nils Wiedemann, Thomas Becker, Nikolaus Pfanner, Martin van der Laan

MOLECULAR BIOLOGY OF THE CELL | AMER SOC CELL BIOLOGY | Published : 2012

Abstract

Mitochondria contain two membranes, the outer membrane and the inner membrane with folded cristae. The mitochondrial inner membrane organizing system (MINOS) is a large protein complex required for maintaining inner membrane architecture. MINOS interacts with both preprotein transport machineries of the outer membrane, the translocase of the outer membrane (TOM) and the sorting and assembly machinery (SAM). It is unknown, however, whether MINOS plays a role in the biogenesis of outer membrane proteins. We have dissected the interaction of MINOS with TOM and SAM and report that MINOS binds to both translocases independently. MINOS binds to the SAM complex via the conserved polypeptide transpo..

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University of Melbourne Researchers

Grants

Awarded by Deutsche Forschungsgemeinschaft, Sonderforschungsbereich


Awarded by Excellence Initiative of the German Federal and State Governments


Awarded by Austrian Science Fund


Awarded by DK Molecular Enzymology


Awarded by Austrian Science Fund (FWF)


Funding Acknowledgements

We thank Peter Rehling for materials and discussion and Anita Kram and Rinse de Boer for assistance with electron microscopy analysis. This work was supported by the Deutsche Forschungsgemeinschaft, Sonderforschungsbereich 746, Excellence Initiative of the German Federal and State Governments (EXC 294 BIOSS; GSC-4 Spemann Graduate School), Bundesministerium fur Bildung und Forschung, Landesforschungspreis Baden-Wurttemberg, and the Austrian Science Fund (project 21429 and DK Molecular Enzymology W901-B05 to G.D.) and was carried out within the research program of the Kluyver Center for Genomics of Industrial Fermentation, which is part of the Netherlands Genomics Initiative/Netherlands Organization for Scientific Research.