Journal article
Dual function of Sdh3 in the respiratory Chain and TIM22 protein translocase of the mitochondrial inner membrane
N Gebert, M Gebert, S Oeljeklaus, K von der Malsburg, DA Stroud, B Kulawiak, C Wirth, RP Zahedi, P Dolezal, S Wiese, O Simon, A Schulze-Specking, KN Truscott, A Sickmann, P Rehling, B Guiard, C Hunte, B Warscheid, M van der Laan, N Pfanner Show all
Molecular Cell | CELL PRESS | Published : 2011
Abstract
The mitochondrial inner membrane harbors the complexes of the respiratory chain and translocase complexes for precursor proteins. We have identified a further subunit of the carrier translocase (TIM22 complex) that surprisingly is identical to subunit 3 of respiratory complex II, succinate dehydrogenase (Sdh3). The membrane-integral protein Sdh3 plays specific functions in electron transfer in complex II. We show by genetic and biochemical approaches that Sdh3 also plays specific functions in the TIM22 complex. Sdh3 forms a subcomplex with Tim18 and is involved in biogenesis and assembly of the membrane-integral subunits of the TIM22 complex. We conclude that the assembly of Sdh3 with differ..
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Awarded by Alexander von Humboldt-Stiftung
Funding Acknowledgements
We are grateful to B. Lemire for antibodies and to T. Becker and C. Mehnert for discussion. This work was supported by the Deutsche Forschungsgemeinschaft, Gottfried Wilhelm Leibniz Program, Excellence Initiative of the German Federal and State Governments (EXC 294 BIOSS; GSC-4 Spemann Graduate School), Bundesministerium fur Bildung und Forschung (Dynamo), Sonderforschungsbereich 746, Landesforschungspreis Baden-Wurttemberg, Trinationales Graduiertenkolleg GRK 1478 (D.A.S.), and an Alexander von Humboldt research fellowship (B.K.).