Two Modular Forms of the Mitochondrial Sorting and Assembly Machinery Are Involved in Biogenesis of alpha-Helical Outer Membrane Proteins
Nicolas Thornton, David A Stroud, Dusanka Milenkovic, Bernard Guiard, Nikolaus Pfanner, Thomas Becker
JOURNAL OF MOLECULAR BIOLOGY | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2010
The mitochondrial outer membrane contains two translocase machineries for precursor proteins--the translocase of the outer membrane (TOM complex) and the sorting and assembly machinery (SAM complex). The TOM complex functions as the main mitochondrial entry gate for nuclear-encoded proteins, whereas the SAM complex was identified according to its function in the biogenesis of beta-barrel proteins of the outer membrane. The SAM complex is required for the assembly of precursors of the TOM complex, including not only the beta-barrel protein Tom40 but also a subset of alpha-helical subunits. While the interaction of beta-barrel proteins with the SAM complex has been studied in detail, little is..View full abstract
Awarded by Trinationales Graduiertenkolleg
Awarded by Sonderforschungsbereich
Awarded by Excellence Initiative of the German Federal & State Governments
This work was supported by the Landesstiftung Baden-Wurttemberg, Deutsche Forschungsgemeinschaft, Trinationales Graduiertenkolleg (GRK 1478), Sonderforschungsbereich 746, Excellence Initiative of the German Federal & State Governments (EXC 294), Landesforschungspreis Baden-Wurttemberg, Gottfried Wilhelm Leibniz Program and Fonds der Chemischen Industrie. We thank Drs. Chris Meisinger, Nils Wiedemann and Mascha Pusnik for discussion and materials.