A Novel Ultra-Stable, Monomeric Green Fluorescent Protein For Direct Volumetric Imaging of Whole Organs Using CLARITY
Daniel J Scott, Natalie J Gunn, Kelvin J Yong, Verena C Wimmer, Nicholas A Veldhuis, Leesa M Challis, Mouna Haidar, Steven Petrou, Ross AD Bathgate, Michael DW Griffin
Scientific Reports | NATURE PUBLISHING GROUP | Published : 2018
Awarded by Australian Research Council Future Fellowship
The authors would like to thank Riley Cridge, Sharon Layfield, and Gabriel Davis Jones for technical assistance, and Riley Metcalfe for protein production. D.J.S. is an NHMRC Boosting Dementia Research Leadership Fellow. R.A.D.B. is an NHMRC Senior Research Fellow. M.D.W.G is the recipient of an Australian Research Council Future Fellowship (project number FT140100544). Studies at the Florey were supported by the Victorian Government's Operational Infrastructure Support Program. Parts of this research were undertaken at the MX2 and SAXS/WAXS beam-lines of the Australian Synchrotron, Victoria, Australia. Crystallisation experiments were performed at the CSIRO Collaborative Crystallisation Centre, Melbourne, Australia.