Journal article

AAV-mediated gene delivery of the calreticulin anti-angiogenic domain inhibits ocular neovascularization

Leilei Tu, Jiang-Hui Wang, Veluchamy A Barathi, Selwyn M Prea, Zheng He, Jia Hui Lee, James Bender, Anna E King, Grant J Logan, Ian E Alexander, Youn-Shen Bee, Ming-Hong Tai, Gregory J Dusting, Bang V Bui, Jingxiang Zhong, Guei-Sheung Liu

ANGIOGENESIS | SPRINGER | Published : 2018

Abstract

Ocular neovascularization is a common pathological feature in diabetic retinopathy and neovascular age-related macular degeneration that can lead to severe vision loss. We evaluated the therapeutic efficacy of a novel endogenous inhibitor of angiogenesis, the calreticulin anti-angiogenic domain (CAD180), and its functional 112-residue fragment, CAD-like peptide 112 (CAD112), delivered using a self-complementary adeno-associated virus serotype 2 (scAAV2) in rodent models of oxygen-induced retinopathy and laser-induced choroidal neovascularization. The expression of CAD180 and CAD112 was elevated in human umbilical vein endothelial cells transduced with scAAV2-CAD180 or scAAV2-CAD112, respecti..

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Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by Natural Science Foundation of Guangdong Province, China


Awarded by Science and Technology Planning Project of Guangdong Province, China


Awarded by Scientific and Cultivation Foundation of the First Affiliated Hospital of Jinan University


Awarded by fundamental Research Funds for the Central Universities


Funding Acknowledgements

This work was supported by grants from The National Health and Medical Research Council of Australia (#1061912), The Natural Science Foundation of Guangdong Province, China (2015A030310158 and 2014A030313359), The Science and Technology Planning Project of Guangdong Province, China (2015B020226003), The Scientific and Cultivation Foundation of the First Affiliated Hospital of Jinan University (2015201), The fundamental Research Funds for the Central Universities (21611446), The Ophthalmic Research Institute of Australia, The Angior Family Foundation and The Rebecca L. Cooper Medical Research Foundation. J.H.W. received a R.B. McComas Research Scholarship in Ophthalmology, a Gordon P. Castles Scholarship and a Melbourne Research Scholarship. G.J.D. received a Principal Research Fellowship from NHMRC. The Centre for Eye Research, Australia, received Operational Infrastructure Support from the Victorian Government.