Biosynthesis of bioactive diterpenoids in the medicinal plant Vitex agnus-castus
Allison M Heskes, Tamil CM Sundram, Berin A Boughton, Niels B Jensen, Nikolaj L Hansen, Christoph Crocoll, Federico Cozzi, Simon Rasmussen, Britta Hamberger, Bjorn Hamberger, Dan Staerk, Birger L Moller, Irini Pateraki
PLANT JOURNAL | WILEY | Published : 2018
Awarded by European Research Council Advanced Grant
Awarded by Danish Innovation Foundation
The authors thank Dan Luo and Qing Liu for assistance with microsomal assays, Codruta Ignea for AM94 S. cerevisiae strain, and the greenhouse personnel, specifically Theodor Emil Bolsterli, at the University of Copenhagen for growing and caring for their plants. The authors gratefully acknowledge Dr David Nelson (University of Tennessee) for CYP naming. This work was supported by the Center for Synthetic Biology (University of Copenhagen Excellence Program for Interdisciplinary Research), by a European Research Council Advanced Grant to BLM (ERC-2012-ADG_20120314), and by the Danish Innovation Foundation funded project 'Plant Power: light-driven synthesis of complex terpenoids using cytochrome P450s' (12-131834; project lead, Dr Poul Erik Jensen, University of Copenhagen). AMH was supported by a Marie Sklodowska Curie Individual Fellowship. TCMS was supported by a SLAI grant from the Ministry of Higher Education Malaysia. MALDI-MSI was conducted at Metabolomics Australia (School of BioSciences, The University of Melbourne, Australia), a NCRIS initiative under Bioplatforms Australia Pty Ltd. The authors declare no competing financial interests.