Effect of interferon γ and tumour necrosis factor α on sensitivity to cisplatin in ovarian carcinoma cell lines

Abstract

This study aimed to investigate whether the biological response modifiers (BRM) interferon γ (IFNγ) and tumour necrosis factor α (TNFα) could enhance the cytotoxic action of cisplatin on ovarian tumour cells in vitro. The sensitivity of four cell lines (OAW42, GG, JAM and PE01) to drugs and drug combinations was tested by a radiolabelled-thymidine incorporation assay. Cell lines demonstrated a range of sensitivity to cisplatin and the innate cytotoxic effect of each of the BRM. When IFNγ was used in combination with cisplatin, a significant enhancement of cisplatin toxicity occurred in three of four cell lines. TNFα demonstrated such an effect in two cell lines but diminished the toxicity of..