Journal article
High Fat Diets Induce Colonic Epithelial Cell Stress and Inflammation that is Reversed by IL-22
Max Gulhane, Lydia Murray, Rohan Lourie, Hui Tong, Yong H Sheng, Ran Wang, Alicia Kang, Veronika Schreiber, Kuan Yau Wong, Graham Magor, Stuart Denman, Jakob Begun, Timothy H Florin, Andrew Perkins, Paraic O Cuiv, Michael A McGuckin, Sumaira Z Hasnain
SCIENTIFIC REPORTS | NATURE PORTFOLIO | Published : 2016
DOI: 10.1038/srep28990
Abstract
AbstractProlonged high fat diets (HFD) induce low-grade chronic intestinal inflammation in mice, and diets high in saturated fat are a risk factor for the development of human inflammatory bowel diseases. We hypothesized that HFD-induced endoplasmic reticulum (ER)/oxidative stress occur in intestinal secretory goblet cells, triggering inflammatory signaling and reducing synthesis/secretion of proteins that form the protective mucus barrier. In cultured intestinal cells non-esterified long-chain saturated fatty acids directly increased oxidative/ER stress leading to protein misfolding. A prolonged HFD elevated the intestinal inflammatory cytokine signature, alongside compromised mucosal barri..
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Awarded by Australian National Health and Medical Research Council
Awarded by National Health and Medical Research Council of Australia
Funding Acknowledgements
We thank Prof. Johannes B. Prins and A/Prof. Jonathan P. Whitehead for their helpful discussion regarding the manuscript. Thank you to Dr. Sandrine Roy for assistance with confocal microscopy and volumetric analysis of images. The authors would also like to thank the staff of the Translational Research Institute and Mater Research Biological Research Facilities for the care of animals. S.Z.H is supported by University of Queensland Postdoctoral Fellowship. M.A.M is supported by Australian National Health and Medical Research Council Principal Research Fellowship. The research was supported by Australian National Health and Medical Research Council Project Grant 1047905,1081473 and the Mater Foundation.