Journal article
ZAP-70 genotype disrupts the relationship between microbiota and host, leading to spondyloarthritis and ileitis in SKG mice
LM Rehaume, S Mondot, D Aguirre De Cárcer, J Velasco, H Benham, SZ Hasnain, J Bowman, M Ruutu, PM Hansbro, MA McGuckin, M Morrison, R Thomas
Arthritis and Rheumatology | WILEY | Published : 2014
DOI: 10.1002/art.38773
Abstract
Objective The spondyloarthritides share genetic susceptibility, interleukin-23 (IL-23) dependence, and the involvement of microbiota. The aim of the current study was to elucidate how host genetics influence gut microbiota and the relationship between microbiota and organ inflammation in spondyloarthritides.Methods BALB/c ZAP-70W163C-mutant (SKG) mice, Toll-like receptor 4 (TLR-4)-deficient SKG mice, and wild-type BALB/c mice were housed under specific pathogen-free conditions. SKG and wild-type BALB/c mice were maintained under germ-free conditions, and some of these mice were recolonized with altered Schaedler flora. All of the mice were injected intraperitoneally with microbial β-1,3-gluc..
View full abstractGrants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
Supported by National Health and Medical Research Council grants 351439 and 569938. Dr. Rehaume's work was supported by a postdoctoral fellowship from the University of Queensland. Dr. McGuckin's work was supported by a Research Fellowship from the National Health and Medical Research Council. Dr. Thomas' work was supported by Arthritis Queensland and by a Future Fellowship from the Australian Research Council.