Journal article

Detection of human metapneumovirus in respiratory secretions by reverse-transcriptasepolymerase chain reaction, indirect imimunofluorescence, and virus isolation in human bronchial epithelial cells

RE Ingram, F Fenwick, R McGuckin, A Tefari, C Taylor, GL Toms

Journal of Medical Virology | WILEY | Published : 2006

Abstract

Over two winters in Newcastle upon Tyne, respiratory secretions, negative by immunofluorescence staining for other respiratory viruses, were tested for the presence of human metapneumovirus (HMPV) by RT-PCR. In the second winter, specimens were also tested by immunofluorescence staining with an anti-HMPV polyclonal rabbit antiserum and immunofluorescence positive specimens were inoculated into a line of human bronchiolar cells, 16HBE140. Overall, 55 of 549 (10%) specimens tested were positive for HMPV by RT-PCR. Of 162 specimens tested by both RT/PCR and immunofluorescence staining, 23 were positive by both techniques. Of five specimens positive by RT-PCR alone, only one was confirmed with a..

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