The Golgi ribbon in mammalian cells negatively regulates autophagy by modulating mTOR activity
Prajakta Gosavi, Fiona J Houghton, Paul J McMillan, Eric Hanssen, Paul A Gleeson
JOURNAL OF CELL SCIENCE | COMPANY BIOLOGISTS LTD | Published : 2018
In vertebrates, individual Golgi stacks are joined into a compact ribbon structure; however, the relevance of a ribbon structure has been elusive. Here, we exploit the finding that the membrane tether of the trans-Golgi network, GCC88 (encoded by GCC1), regulates the balance between Golgi mini-stacks and the Golgi ribbon. Loss of Golgi ribbons in stable cells overexpressing GCC88 resulted in compromised mechanistic target of rapamycin (mTOR) signaling and a dramatic increase in LC3-II-positive autophagosomes, whereas RNAi-mediated depletion of GCC88 restored the Golgi ribbon and reduced autophagy. mTOR was absent from dispersed Golgi mini-stacks whereas recruitment of mTOR to lysosomes was u..View full abstract
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Awarded by Australian Research Council
This work was supported by funding from the Australian Research Council (DP160102394).