Rapid recovery of nuclear and mitochondrial genes by genome skimming from Northern Hemisphere freshwater crayfish
Frederic Grandjean, Mun Hua Tan, Han Ming Gan, Yin Peng Lee, Tadashi Kawai, Robert J Distefano, Martin Blaha, Angela J Roles, Christopher M Austin
ZOOLOGICA SCRIPTA | WILEY | Published : 2017
Molecular phylogenetics has benefited tremendously from the advent of next-generation sequencing, enabling quick and cost-effective recovery of whole mitogenomes via an approach referred to as ‘genome skimming’. Recently, genome skimming has been utilised to recover highly repetitive nuclear genes such as 18S and 28S ribosomal RNA genes that are useful for inferring deeper evolutionary relationships. To address some outstanding issues in the relationships among Northern Hemisphere freshwater crayfish (Astacoidea), we sequenced the partial genome of crayfish species from Asian, North American and European genera and report the successful recovery of whole mitogenome sequences in addition to t..View full abstract
Funding for this study was provided by the Monash University Malaysia Tropical Medicine and Biology Platform. MHT and HMG would like to acknowledge the Monash University Malaysia High Performance Computing infrastructure for the provision of computational resources.