Journal article
Etiology matters-Genomic DNA Methylation Patterns in Three Rat Models of Acquired Epilepsy
KJ Debski, A Pitkanen, N Puhakka, AM Bot, I Khurana, KN Harikrishnan, M Ziemann, A Kaspi, A El-Osta, K Lukasiuk, K Kobow
Scientific Reports | NATURE PORTFOLIO | Published : 2016
DOI: 10.1038/srep25668
Abstract
This study tested the hypothesis that acquired epileptogenesis is accompanied by DNA methylation changes independent of etiology. We investigated DNA methylation and gene expression in the hippocampal CA3/dentate gyrus fields at 3 months following epileptogenic injury in three experimental models of epilepsy: focal amygdala stimulation, systemic pilocarpine injection, or lateral fluid-percussion induced traumatic brain injury (TBI) in rats. In the models studies, DNA methylation and gene expression profiles distinguished controls from injured animals. We observed consistent increased methylation in gene bodies and hypomethylation at non-genic regions. We did not find a common methylation sig..
View full abstractGrants
Awarded by Polish Ministry of Science and Higher Education
Awarded by National Science Centre, Poland
Awarded by German Research Foundation (DFG) within the EUROCORES Programme EuroEPINOMICS/EpiGENet of the European Science Foundation
Funding Acknowledgements
This work was supported by Polish Ministry of Science and Higher Education grant 888/N-ESF-EuroEPINOMICS/10/2011/0 (AB, KD, KL); the National Science Centre, Poland grant 2014/13/N/NZ2/00587 (KD); the Academy of Finland ESF-EuroEPINOMICS/2012 grant (AP), the University of Eastern Finland Spearhead Funding (AP); and the German Research Foundation (DFG, grant BL 421/3-1) within the EUROCORES Programme EuroEPINOMICS/EpiGENet of the European Science Foundation (KK).