Journal article

The NS segment of H5N1 avian influenza viruses (AIV) enhances the virulence of an H7N1 AIV in chickens

Julia Vergara-Alert, Nuria Busquets, Maria Ballester, Aida J Chaves, Raquel Rivas, Roser Dolz, Zhongfang Wang, Stephan Pleschka, Natalia Majo, Fernando Rodriguez, Ayub Darji

VETERINARY RESEARCH | BMC | Published : 2014

Abstract

Some outbreaks involving highly pathogenic avian influenza viruses (HPAIV) of subtypes H5 and H7 were caused by avian-to-human transmissions. In nature, different influenza A viruses can reassort leading to new viruses with new characteristics. We decided to investigate the impact that the NS-segment of H5 HPAIV would have on viral pathogenicity of a classical avian H7 HPAIV in poultry, a natural host. We focussed this study based on our previous work that demonstrated that single reassortment of the NS-segment from an H5 HPAIV into an H7 HPAIV changes the ability of the virus to replicate in mammalian hosts. Our present data show that two different H7-viruses containing an NS-segment from H..

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University of Melbourne Researchers

Grants

Awarded by Spanish Government


Awarded by FPI-MICINN


Awarded by German Research Foundation (DFG)


Awarded by German Ministry for Education and Research (BMBF)


Funding Acknowledgements

The authors thank M. Perez, R. Valle, B. Vidana and the personnel of the BSL-3 of CReSA for their excellent technical assistance. We also want to express our gratitude to Dr Mikhail Matrosovich for his critical reading and scientific discussion. This work was supported by the Spanish Government Grants AGL2007-60434/GAN and AGL2010-22229-C03-01 (Ministry of Science and Innovation, MICINN). J.V-A was supported by FPI-MICINN Training Grant BES-2008-00260. This work was also supported in part by grants from the German Research Foundation (DFG grant GRK1384, fellowship to Z.W.) and from the German Ministry for Education and Research funded "FluResearch-Net-Molecular Signatures Determining Pathogenicity and Species Transmission of Influenza A Viruses" (BMBF grant 01 KI 07136, to S.P.).