Journal article

NS Reassortment of an H7-Type Highly Pathogenic Avian Influenza Virus Affects Its Propagation by Altering the Regulation of Viral RNA Production and Antiviral Host Response

Zhongfang Wang, Nicole C Robb, Eva Lenz, Thorsten Wolff, Ervin Fodor, Stephan Pleschka

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2010

Abstract

Highly pathogenic avian influenza viruses (HPAIV) with reassorted NS segments from H5- and H7-type avian virus strains placed in the genetic background of the A/FPV/Rostock/34 HPAIV (FPV; H7N1) were generated by reverse genetics. Virological characterizations demonstrated that the growth kinetics of the reassortant viruses differed from that of wild-type (wt) FPV and depended on whether cells were of mammalian or avian origin. Surprisingly, molecular analysis revealed that the different reassortant NS segments were not only responsible for alterations in the antiviral host response but also affected viral genome replication and transcription as well as nuclear ribonucleoprotein (RNP) export...

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Funding Acknowledgements

This work was supported in part by grants from the DFG (GRK1384, fellowship to Z.W.), from the European Specific Targeted Research Project, EuroFlu-Molecular Factors and Mechanisms of Transmission and Pathogenicity of Highly Pathogenic Avian Influenza Virus, funded by the 6th Framework Program (FP6) of the EU (grant SP5B-CT-2007-044098, to S.P.), from the FluResearchNet-Molecular Signatures Determining Pathogenicity and Species Transmission of Influenza A Viruses (grant 01 KI 07136, to S.P., and grant 01 KI 07132, to T.W.), and from the MRC (grant G0700848, to E.F.).