Reverse engineering the antigenic architecture of the haemagglutinin from influenza H5N1 clade 1 and 2.2 viruses with fine epitope mapping using monoclonal antibodies
Steve Rockman, Sarina Camuglia, Kirsten Vandenberg, Chi Ong, Mark A Baker, Roger L Nation, Jian Li, Tony Velkov
MOLECULAR IMMUNOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2013
The induction of neutralising antibodies to the viral surface glycoprotein, haemagglutinin (HA) is considered the cornerstone of current seasonal and pandemic influenza vaccines. Mapping of neutralising epitopes using monoclonal antibodies (mAbs) helps define mechanisms of antigenic drift, neutralising escape and facilitates pre-pandemic vaccine design. In the present study we reverse engineered the antigenic structure of the HAs of two highly pathogenic H5N1 vaccine strains representative of currently circulating clade 1 and 2.2 H5N1 viruses. The HA sequence of the A/Vietnam/1194/04 clade 1 virus was progressively mutated into the HA sequence of the clade 2.2 virus, A/Bar-headed Goose/Qingh..View full abstract
Awarded by National Health and Medical Research Council of Australia
J.L. is an Australian NHMRC Senior Research Fellow. T.V. and M.B are Australian NHMRC Career Development Research Fellows. This work was supported by the strategic award grant 594875 from the National Health and Medical Research Council of Australia.