Journal article

A reliable and easy to transport quality control method for chlamydia and gonorrhoea molecular point of care testing

SG Badman, LM Causer, R Guy, H Wand, B Donovan, SN Tabrizi, D Speers, MD Shephard, A Vallely, D Whiley

Pathology | ELSEVIER | Published : 2018

Abstract

Quality control (QC) is an essential component of point-of-care testing programs. In the context of a randomised-controlled trial (TTANGO) using GeneXpert (Xpert) Chlamydia trachomatis and Neisseria gonorrhoeae (CT/NG) point-of-care testing in remote areas of Australia, we aimed to develop and utilise a stable positive control material. Bacterial cultures of CT and NG were resuspended together to provide cycle threshold (Ct) values of approximately 25 cycles for both CT and NG when tested on the Xpert CT/NG assay. These positive control suspensions were dried in aliquots, heat inactivated, and then provided to 12 participating health services as research-only QC samples in kit form. At each ..

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University of Melbourne Researchers

Grants

Awarded by National Health and Medical Research Council (NHMRC)


Awarded by NHMRC


Funding Acknowledgements

The TTANGO Trial was funded by a National Health and Medical Research Council (NHMRC) project grant #109902 and an NHMRC partnership project grant # GNT1092503. The authors state that there are no conflicts of interest to disclose.