Journal article

In vitro maintenance and retroviral transduction of human myeloma cells in long-term marrow cultures.

AK Stewart, HM Prince, D Cappe, P Chu, C Lutzko, DR Sutherland, ID Dubé

Cancer Gene Therapy | Published : 1997

Abstract

One objective of clinical gene marking trials in multiple myeloma (MM) is to determine the extent to which relapse after stem cell transplant is attributable to contamination of the autograft with myeloma cells. A requirement in these studies is ex vivo genetic marking of malignant cells present in autografts which are derived from patients exposed to significant prior chemotherapy. We evaluated gene marking of cloonogenic myeloma cells in marrow aspirates from 14 patients with MM. To effect gene transfer we utilized a long-term marrow culture (LTMC) system previously shown to facilitate gene transfer into a spectrum of hematopoietic progenitor and stem cells. Transduction of cells in LTMC w..

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