The role of Angiotensin II/AT1 receptor signaling in regulating retinal microglial activation

JA Phipps; KA Vessey; A Brandli; N Nag; MX Tran; AI Jobling; EL Fletcher

Journal article

The role of Angiotensin II/AT1 receptor signaling in regulating retinal microglial activation

JA Phipps, KA Vessey, A Brandli, N Nag, MX Tran, AI Jobling, EL Fletcher

Investigative Ophthalmology and Visual Science | Published : 2018

DOI: 10.1167/iovs.17-22416

Abstract

PURPOSE. This study explored whether the proangiogenic factor Angiotensin II (AngII) had a direct effect on the activation state of microglia via the Angiotensin type 1 receptor (AT1-R). METHODS. Microglial dynamic activity was investigated in live retinal flatmounts from adult Cx3Cr1+/GFP mice under control, AngII (5 μM) or AngII (5μM) + candesartan (0.227μM) conditions. The effects of intravitreal administration of AngII (10 mM) were also investigated at 24 hours, with retinae processed for immunocytochemistry, flow cytometry, or inflammatory quantitative PCR arrays. RESULTS. We found FACS isolated retinal microglia expressed AT1-R. In retinal flatmounts, microglia showed characteristic mo..

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University of Melbourne Researchers

Kirstan Vessey Author

Alice Brandli Author

Nupur Nag Author

Andrew Jobling Author

Related Projects (1)

A NOVEL CAUSE OF VISION LOSS

This project will examine a novel way that photoreceptors in the eye are regulated. In particular, the communication between resident immune..

Grants

Awarded by Australian Research Council

Funding Acknowledgements

Supported by ARC Discovery Early Career Research Fellowship (DE140100099; JAP) and National Health and Medical Research Council of Australia (1061418; ELF and AIJ).