Localization of GABAA receptors in the rat retina.
U Greferath, F Müller, H Wässle, B Shivers, P Seeburg
Vis Neurosci | Published : 1993
Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian retina. The present paper describes the localization of GABAA receptors in the rat retina as revealed by in situ hybridization and immunocytochemistry. In situ hybridization with probes against various alpha subunits revealed a marked differential expression pattern. The alpha 1 subunit gene is expressed mainly in the bipolar and horizontal cell layer, the alpha 2 gene in the amacrine and ganglion cell layer, and the alpha 4 gene in a subpopulation of amacrine cells. beta subunit mRNA is present diffusely throughout the entire inner nuclear layer and in the ganglion cell layer. The monoclonal antibody bd..View full abstract