Structural basis of autoregulation of phenylalanine hydroxylase.
B Kobe, IG Jennings, CM House, BJ Michell, KE Goodwill, BD Santarsiero, RC Stevens, RG Cotton, BE Kemp
Nat Struct Biol | Published : 1999
Phenylalanine hydroxylase converts phenylalanine to tyrosine, a rate-limiting step in phenylalanine catabolism and protein and neurotransmitter biosynthesis. It is tightly regulated by the substrates phenylalanine and tetrahydrobiopterin and by phosphorylation. We present the crystal structures of dephosphorylated and phosphorylated forms of a dimeric enzyme with catalytic and regulatory properties of the wild-type protein. The structures reveal a catalytic domain flexibly linked to a regulatory domain. The latter consists of an N-terminal autoregulatory sequence (containing Ser 16, which is the site of phosphorylation) that extends over the active site pocket, and an alpha-beta sandwich cor..View full abstract