Journal article
Ablation of Grb10 Specifically in Muscle Impacts Muscle Size and Glucose Metabolism in Mice
LJ Holt, AE Brandon, L Small, E Suryana, E Preston, D Wilks, N Mokbel, CA Coles, JD White, N Turner, RJ Daly, GJ Cooney
Endocrinology | OXFORD UNIV PRESS INC | Published : 2018
Abstract
Grb10 is an adaptor-type signaling protein most highly expressed in tissues involved in insulin action and glucose metabolism, such as muscle, pancreas, and adipose. Germline deletion of Grb10 in mice creates a phenotype with larger muscles and improved glucose homeostasis. However, it has not been determined whether Grb10 ablation specifically in muscle is sufficient to induce hypermuscularity or affect whole body glucose metabolism. In this study we generated muscle-specific Grb10-deficient mice (Grb10-mKO) by crossing Grb10 flox/flox mice with mice expressing Cre recombinase under control of the human α-skeletal actin promoter. One-year-old Grb10-mKO mice had enlarged muscles, with greate..
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Awarded by French Muscular Dystrophy Association
Funding Acknowledgements
L.J.H. and N.M. were supported by NHMRC Grant 1009794. G.J.C. and R.J.D. were supported by research fellowships from the NHMRC. N.T. was supported by an Australian Research Council Future Fellowship. L.S. was supported by an Australian Postgraduate Award. A.E.B, E.S., E.P., and D.W. were supported by NHMRC program Grant 53921. J.D.W. and C.A.C. were supported by Muscular Dystrophy Australia, Murdoch Children's Research Institute, and Victorian Government's Operational Infrastructure Support Program. The contents of the published material are solely the responsibility of the Garvan Institute of Medical Research and do not reflect the views of the NHMRC.