Quantitative analysis of the developing rat kidney: absolute and relative volumes and growth curves.
JF Bertram, RJ Young, K Spencer, I Gordon
Anat Rec | Published : 2000
The development of the permanent kidney, or metanephros, is a complex process. In the present study, stereological methods were used at the light microscopic level to estimate the absolute volumes and volume densities of seven compartments in the developing rat metanephros, from embryonic day 14 (E14) to E21. Metanephroi from time-mated Sprague-Dawley rats were embedded whole in glycolmethacrylate, exhaustively sectioned at 2 microm and stained with PAS. The left metanephros from three embryos from each of three mothers were analysed at each of the ages (a total of 72 metanephroi). Relative volumes were multiplied by total metanephric volume to obtain absolute volumes. Total metanephric volu..View full abstract