Journal article

Characterization of Diffuse Intrinsic Pontine Glioma Radiosensitivity using Synchrotron Microbeam Radiotherapy and Conventional Radiation Therapy In Vitro

LM Smyth, PAW Rogers, JC Crosbie, JF Donoghue



Synchrotron microbeam radiation therapy is a promising preclinical radiotherapy modality that has been proposed as an alternative to conventional radiation therapy for diseases such as diffuse intrinsic pontine glioma (DIPG), a devastating pediatric tumor of the brainstem. The primary goal of this study was to characterize and compare the radiosensitivity of two DIPG cell lines (SF7761 and JHH-DIPG-1) to microbeam and conventional radiation. We hypothesized that these DIPG cell lines would exhibit differential responses to each radiation modality. Single cell suspensions were exposed to microbeam (112, 250, 560, 1,180 Gy peak dose) or conventional (2, 4, 6 and 8 Gy) radiation to produce clon..

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Awarded by National Health and Medical Research Council (NHMRC) of Australia

Awarded by NHMRC

Funding Acknowledgements

Support was provided by an Australian Government Research Training Project Scholarship (LMLS). JCC was previously supported by an Early Career Research Fellowship from the National Health and Medical Research Council (NHMRC) of Australia (no. 1036174). This project was supported by grants from the Robert Connor Dawes Foundation, the Isabella and Marcus Foundation and the NHMRC (Project Grant No. 1061772). We thank Jayde Livingstone, Andrew Stevenson and the team at the IMBL for their assistance in irradiations and dosimetry at the Australian Synchrotron, Liam Day for performing Monte Carlo simulations of MRT dosimetry, Premila Paiva for assistance with dose equivalence calculations and Sandy Fung at the Monash Institute of Pharmaceutical Sciences Flow Cytometry Facility.