Journal article

Biologically active constituents of the secretome of human W8B2( ) cardiac stem cells

Shuai Nie, Xin Wang, Priyadharshini Sivakumaran, Mark MW Chong, Xin Liu, Tara Karnezis, Nadeeka Bandara, Kaloyan Takov, Cameron J Nowell, Stephen Wilcox, Mitch Shambrook, Andrew F Hill, Nicole C Harris, Andrew E Newcomb, Padraig Strappe, Ramin Shayan, Damian Hernandez, Jordan Clarke, Eric Hanssen, Sean M Davidson Show all

SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2018

Abstract

The benefits of adult stem cells for repair of the heart have been attributed to the repertoire of salutary paracrine activities they appear to exert. We previously isolated human W8B2+ cardiac stem cells (CSCs) and found they powerfully influence cardiomyocytes and endothelial cells to collectively promote cardiac repair and regeneration. Here, the complexity of the W8B2+ CSC secretomes was characterised and examined in more detail. Using ion exchange chromatography to separate soluble proteins based on their net surface charge, the secreted factors responsible for the pro-survival activity of W8B2+ CSCs were found within the low and medium cation fractions. In addition to the soluble prote..

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Grants

Funding Acknowledgements

This work was carried out with support from the St Vincent's Hospital (Melbourne) Research Endowment Fund, CASS foundation and Stafford Fox Medical Research Foundation. Alice Pebay is an Australian Research Council Future Fellow. Joshua Ho is a National Health and Medical Research Council Career Development Fellow and a National Heart Foundation Future Leader Fellow. The O'Brien Institute Department, St Vincent's Institute of Medical Research, and the Centre for Eye Research Australia receive Operational Infrastructure Support from the Victorian State Government's Department of Innovation, Industry and Regional Development.