Dot/Icm-Translocated Proteins Important for Biogenesis of the Coxiella burnetii-Containing Vacuole Identified by Screening of an Effector Mutant Sublibrary
Emerson Crabill, Whitman B Schofield, Hayley J Newton, Andrew L Goodman, Craig R Roy
INFECTION AND IMMUNITY | AMER SOC MICROBIOLOGY | Published : 2018
Coxiella burnetii is an intracellular pathogen that replicates in a lysosome-derived vacuole. A determinant necessary for C. burnetii virulence is the Dot/Icm type IVB secretion system (T4SS). The Dot/Icm system delivers more than 100 proteins, called type IV effectors (T4Es), across the vacuolar membrane into the host cell cytosol. Several T4Es have been shown to be important for vacuolar biogenesis. Here, transposon (Tn) insertion sequencing technology (INSeq) was used to identify C. burnetii Nine Mile phase II mutants in an arrayed library, which facilitated the identification and clonal isolation of mutants deficient in 70 different T4E proteins. These effector mutants were screened in H..View full abstract
Awarded by National Institutes of Health (NIH)
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
This work was funded by the National Institutes of Health (NIH) (F32 GM 108411 R01 AI 114760 and R35 GM118159). A.L.G. is supported by the Burroughs Wellcome Fund and the HHMI Faculty Scholars Program.