Journal article
Whole genome sequencing of candida glabrata for detection of markers of antifungal drug resistance
C Biswas, SCA Chen, C Halliday, E Martinez, RJ Rockett, Q Wang, VJ Timms, R Dhakal, R Sadsad, KJ Kennedy, G Playford, DJ Marriott, MA Slavin, TC Sorrell, V Sintchenko
Journal of Visualized Experiments | JOURNAL OF VISUALIZED EXPERIMENTS | Published : 2017
DOI: 10.3791/56714
Abstract
Candida glabrata can rapidly acquire mutations that result in drug resistance, especially to azoles and echinocandins. Identification of genetic mutations is essential, as resistance detected in vitro can often be correlated with clinical failure. We examined the feasibility of using whole genome sequencing (WGS) for genome-wide analysis of antifungal drug resistance in C. glabrata. The aim was torecognize enablers and barriers in the implementation WGS and measure its effectiveness. This paper outlines the key quality control checkpoints and essential components of WGS methodology to investigate genetic markers associated with reduced susceptibility to antifungal agents. It also estimates t..
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Funding Acknowledgements
This work was supported by the Centre for Infectious Diseases and Microbiology, Public Health. The authors have not received any other funding for this study. The authors thank Drs Alicia Arnott, Nathan Bachmann and Ranjeeta Menon for their expert advice and assistance with the whole genome sequencing experiment.