Journal article

Extracellular vesicles from early stage Plasmodium falciparum-infected red blood cells contain PfEMP1 and induce transcriptional changes in human monocytes

Natalia G Sampaio, Samantha J Emery, Alexandra L Garnham, Qiao Y Tan, Xavier Sisquella, Matthew A Pimentel, Aaron R Jex, Neta Regev-Rudzki, Louis Schofield, Emily M Eriksson



Pathogens can release extracellular vesicles (EVs) for cell-cell communication and host modulation. EVs from Plasmodium falciparum, the deadliest malaria parasite species, can transfer drug resistance genes between parasites. EVs from late-stage parasite-infected RBC (iRBC-EVs) are immunostimulatory and affect endothelial cell permeability, but little is known about EVs from early stage iRBC. We detected the parasite virulence factor PfEMP1, which is responsible for iRBC adherence and a major contributor to disease severity, in EVs, only up to 12-hr post-RBC invasion. Furthermore, using PfEMP1 transport knockout parasites, we determined that EVs originated from inside the iRBC rather than th..

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Awarded by NHMRC Dora Lush Scholarship

Awarded by National Health and Medical Research Council

Funding Acknowledgements

Victorian State Government Operational Infrastructure Support and Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme; NHMRC Dora Lush Scholarship, Grant/Award Number: APP1038030; National Health and Medical Research Council, Grant/Award Numbers: APP106722 and APP1126395