Journal article

DExSI: a new tool for the rapid quantitation of C-13-labelled metabolites detected by GC-MS

Michael J Dagley, Malcolm J McConville

BIOINFORMATICS | OXFORD UNIV PRESS | Published : 2018

Abstract

Summary: Stable isotope directed metabolomics is increasingly being used to measure metabolic fluxes in microbial, plant and animal cells. Incorporation of 13C/15N isotopes into a wide range of metabolites is typically determined using gas chromatography-mass spectrometry (GC/MS) or other hyphenated mass spectrometry approaches. The DExSI (Data Extraction for Stable Isotope-labelled metabolites) pipeline is an interactive graphical software package which can be used to rapidly quantitate isotopologues for a wide variety of metabolites detected by GC/MS. DExSI performs automated metabolite annotation, mass and positional isotopomer abundance determination and natural isotope abundance correct..

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