Journal article

FORMATION OF PROLINE METABOLITES IN CHICK-EMBRYO BONE - INTERFERENCE WITH THE MEASUREMENT OF FREE HYDROXYPROLINE BY ION-EXCHANGE CHROMATOGRAPHY

M CHOJKIER, J BATEMAN, JM PHANG, B PETERKOFSKY

ANALYTICAL BIOCHEMISTRY | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS | Published : 1982

Abstract

Metabolites of l-[14C]proline were found in the trichloroacetic acid-soluble fraction of 16-day-old chick embryo frontal bones. In several ion-exchange procedures these metabolites interfered with the analysis of hydroxyproline derived from the metabolic breakdown of collagen. The major metabolite was identified as glutamic acid by its chromatographic and crystallization properties. It was eluted from AG50 cation-exchange resin with 1.0 n HCL in the hydroxyproline region, but was separated from hydroxyproline on a DC-6A column in the amino acid analyzer. Another metabolite was identified as aspartic acid. It was not separated from hydroxyproline on either AG50 using 1 n HCL for elution or on..

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