Journal article

UNCL, the mammalian homologue of UNC-50, is an inner nuclear membrane RNA-binding protein

J Fitzgerald, D Kennedy, N Viseshakul, BN Cohen, J Mattick, JF Bateman, JR Forsayeth

BRAIN RESEARCH | ELSEVIER SCIENCE BV | Published : 2000

Abstract

We isolated a mammalian homologue of the C. elegans gene unc-50 that we have named UNCL. The 777 kb rat UNCL cDNA encodes a 259 amino acid protein that is expressed in a wide variety of tissues with highest mRNA levels in brain, kidney and testis. Hydropathy plot analysis and in vitro translation experiments with microsomal membranes indicate that UNCL is a transmembrane protein. Hemagglutinin tagged UNCL was stably transfected into SaOS-2 osteosarcoma cells and exhibited a nuclear rim staining pattern which was retained following extraction with 1% Triton X-100, suggesting that UNCL localizes to the inner nuclear membrane. UNCL-HA was extractable in 350 mM NaCl, suggesting that UNCL is not ..

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