CALBINDIN-D-28K LIKE IMMUNOREACTIVITY IN SUPERFICIAL DORSAL HORN NEURONS AND EFFECTS OF SCIATIC CHRONIC CONSTRICTION INJURY
MJ Stebbing, S Balasubramanyan, PA Smith
NEUROSCIENCE | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2016
The neuropathic pain that results from peripheral nerve injury is associated with alterations in the properties of neurons in the superficial spinal laminae. Chronic constriction injury (CCI) of the rat sciatic nerve increases excitatory synaptic drive to excitatory neurons in the substantia gelatinosa while limiting that to inhibitory neurons. Since the calcium-binding protein calbindin D-28K has been associated with excitatory neurons, we examined whether CCI altered the properties of neurons expressing calbindin-like immunoreactivity (Cal+). These account for 30% of the neurons in lamina I and II. Calbindin did not co-localize with any particular electrophysiological phenotype of neuron; ..View full abstract
Awarded by Canadian Institutes of Health Research (CIHR)
Supported by the Canadian Institutes of Health Research (CIHR; Funding reference # 81089). RMIT travel award and Neuroscience Victoria (Australia). We thank Professor John Furness for use of confocal microscope facilities at the University of Melbourne.