Journal article
Effects of C1 inhibitor on endothelial cell activation in a rat hind limb ischemia-reperfusion injury model
S Zhang, J Shaw-Boden, Y Banz, AK Bongoni, A Taddeo, R Spirig, MW Nolte, PJ Cowan, R Rieben
Journal of Vascular Surgery | MOSBY-ELSEVIER | Published : 2018
Abstract
Objective: Ischemia-reperfusion (I/R) injury is a major clinical problem linked to vascular surgery. Currently, no drugs to prevent or to treat I/R injury are approved for clinical use. C1 inhibitor (C1 INH) is known to reduce activation of the plasma cascade systems that are involved in the pathophysiologic process of I/R injury. The aim of this study was therefore to investigate the effect of C1 INH on complement deposition and endothelial cell activation in a rat model of hind limb I/R injury. Methods: Male Wistar rats (wild type, bred at the central animal facility, University of Bern), weighing 250 to 320 g, were used. The rats underwent 2-hour ischemia and 24-hour reperfusion by unilat..
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Funding Acknowledgements
The authors would like to thank Claudia Duhrkop for helpful discussions and support with the experiments. We also thank the late Sidney Shaw for critical revision of the manuscript. Sylvia Miescher (CSL Behring) provided C1 inhibitor and vehicle buffer, and she helped us with critical feedback and discussions. This work was supported by the Microscopy Imaging Center, University of Bern, as well as by the Translational Research Unit, Institute of Pathology, University of Bern.