Journal article
Superior properties of CellTrace Yellow™ as a division tracking dye for human and murine lymphocytes
JC Tempany, JHS Zhou, PD Hodgkin, VL Bryant
Immunology and Cell Biology | WILEY | Published : 2018
DOI: 10.1111/imcb.1020
Abstract
The discovery of cell division tracking properties of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) by Lyons and Parish in 1994 led to a broad range of new methods and numerous important biological discoveries. After labeling, CFSE is attached to free amine groups and intracellular proteins in the cytoplasm and nucleus of a cell, and halves in fluorescence intensity with each round of cell division, enabling enumeration of the number of divisions a cell has undergone. A range of popular division tracking dyes were subsequently developed, including CellTrace Violet (CTV), making available the green fluorescent channel previously occupied by CFSE. More recently, CellTrace Ye..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
We thank Susanne Heinzel for critical review of the manuscript. We thank the Australian National Health and Medical Research Council (NHMRC, Program Grant 1054925, Project Grant 1127198) and Independent Research Institutes Infrastructure Support Scheme Grant 361646 for grant support. We also receive support from the Victorian State Government Operational Infrastructure Scheme, Rae grant, Bloody Long Way (BLW) and Walter & Eliza Hall Institute Innovation Grant. JCT is supported by an Australian Government Research Training Scholarship. JHSZ is the recipient of an Australian Postgraduate Award. We would like to thank Thermo Fisher Scientific for providing trial samples of CTY and CTFR upon request. We have no personal or financial affiliation with Thermo Fisher Scientific.